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Genetic Transformation using Agrobacterium rhizogenes for the Production of Valuable Anti-Cancer Compound, Withaferin-A from Withania somnifera (L.) Dunal

Authors

  • C.Thilip Department of Botany,Periyar Univesity Cosntituent College of Arts and Science,Sendamangalam –637409,Namakkal –Dt
  • P. Raja Plant Molecular Biology Laboratory, Department of Botany,Jamal Mohamed College, Tiruchirappalli-620 020
  • K.Mohamed Rafi Plant Molecular Biology Laboratory, Department of Botany,Jamal Mohamed College, Tiruchirappalli-620 020
  • K.P.Faizal Plant Molecular Biology Laboratory, Department of Botany,Jamal Mohamed College, Tiruchirappalli-620 020
  • G.Thiagu Plant Molecular Biology Laboratory, Department of Botany,Jamal Mohamed College, Tiruchirappalli-620 020
  • A.Aslam Plant Molecular Biology Laboratory, Department of Botany,Jamal Mohamed College, Tiruchirappalli-620 020
  • A. Shajahan Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli - 620 020, Tamil Nadu.

DOI:

https://doi.org/10.46947/jarj1120201

Keywords:

Ashwagandha; Physical leaf traits; Withaferin-A; Agrobacterium rhizogenes; Sonication; Hairy roots.

Abstract

Withania somnifera (L.) Dunal is an important ayurvedic medicinal plant and its primary bioactive compound, withaferin-A possess several pharmaceutical and therapeutic values ranging from immunomodulation to anti-cancer property. For commercial production of withaferin-A, field grown plant materials have generally been used but the quality and uniformity in the levels of active constituents are highly affected by genotype and environmental conditions. Plant tissue culture methods are best alternative way to enhance commercial production of withaferin-A. In this present study, we summarize our recent findings on characterization and screening of wild accessions of W. somnifera collected from Tamil Nadu state. Further, an improved Agrobacterium rhizogenes mediated hairy root culture system was developed and this system providing an efficient tool attaining better transformation efficiency and useful for commercial in vitro production of withanolides.

References

Bhattacharaya SK, Bhattacharya D, Sairam K, Ghosal S (2002) Effect of Withania somnifera glycowithanolides on rat model of tardie dyskinesia. Pytomedicine 9: 167-170. https://doi.org/10.1078/0944-7113-00089

Thilip C, Soundar Raju C, Varutharaju K, Aslam A, Shajahan A (2015A) Establishment of adventitious root culture from cell suspensions of Withania somnifera (L.) Dunal:an in vitro approach for production of withanolide. International journal of pharma and Bio Sciences 6: 1030-1037.

Hu ZB, Du M (2006) Hairy root and its application in plant genetic engineering. Journal of Integrative Plant Biology 48: 121-127. https://doi.org/10.1111/j.1744-7909.2006.00121.x

Saravanakumar A, Aslam A, Shajahan A (2012) Development and optimization of hairy root culture systems in Withania somnifera (L.) Dunal of withaferin-A production. African journal of Biotechnology 11 (98): 16412-16420.

Aslam A, Mohamed Rafi K, Kathiravan K, Shajahan A (2010) Class-based stratification matrix for physical leaf traits in phenetic relations of Withania somnifera (L.) Dunal accessions. Plant Systematics and Evolution 288: 99-111. https://doi.org/10.1007/s00606-010-0316-z

Kumar A, Kaul MK, Bhan MK, Khanna PK, Suri KA (2007) Morphological and chemical variation in 25 collections of the Indian medicinal plant Withania somnifera (L.) Dunal (Solanaceae). Genetic Resources and Crop Evolution 54: 655-660. https://doi.org/10.1007/s10722-006-9129-x

Thilip C, Soundar Raju C, Varutharaju K, Aslam A, Shajahan A (2015) Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment. 3 Biotech 5: 949-956. https://doi.org/10.1007/s13205-015-0297-2

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Published

2020-02-10

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Issue

Vol. 1 No. 1 (2020): Jamal Academic Research Journal-An Interdisciplinary (JARJ)

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